The Extraction and Purification of DNA.
Deoxyribonucleic Acid, commonly referred to as DNA, is the molecule found in everything termed as living. This molecule is known to contain a genetic code for every organism hence it is a blueprint that continuously acts in plans of any substance that is produced by these organisms at the course of their living. As much as the molecule has a popular usage, very few individuals have actually seen this molecule; actually the molecule happens to be too small for one to see. However, one can simply do extraction on the molecules easily at home or in the school using available household materials like plants and especially peas.
Some of the crucial materials that you may require for the process include alcohol, meat tenderizer, dishwashing detergent mostly in liquid form, a blender, a beaker or rather clear glass container, a stirring rod or a spoon. To begin with, blend at least a half cup of split peas in a cupful of water. Having obtained a soupy mixture from the blend, use a homogenous solution to separate the mixture. One may also consider keeping the extra solution just in case. After obtaining the solution, consider pouring some of it into a container up to a level of at least two inches, the solution used here should be adequate.
In the third step, you need to add at least a tablespoonful of the liquid dishwashing detergent on top of the solution. Again stir the mixture slowly for about five minutes. In the next procedure; add a pinch of the meat tenderizer on the mixture then stir for another five minutes. Having stirred the mixture, and then drizzle some alcohol above it. Consider adding the alcohol until you obtain a layer of three inches on the split peas solution. Peas are mostly used for this extraction because they are so rich in the molecule. Again the detergent is used to break down the membrane cell for it to release its contents and the meat tenderizer to dissolve the membrane nuclear for it to release the DNA inside. The alcohol is used in the process to bring out the DNA strands.
The purification procedure can utilize various processing kits. This is because, different samples work with different kind of tissues, forensic samples, plant parts and also cellular structure. Continuously, there are new inventions being made on machines and cutting edge products through different forms of amplification and facilitations for use in new projects and experiments. One such way involves use of magnetized beads which happens to be very effective. Another way involves the use of plasmid purification kits which is able to give low endotoxin levels for use in the subject transfection.